anti ifnλ2 3 antibody Search Results


anti ifnλ2 3 antibody  (R&D Systems)
94
R&D Systems anti ifnλ2 3 antibody
Anti Ifnλ2 3 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+ifn%CE%BB2+3+antibody/Mouse+IL-28A%2FB+(IFN-lambda+2%2F3)+Antibody/pmc09558022-142-28-33
Average 94 stars, based on 1 article reviews
anti ifnλ2 3 antibody - by Bioz Stars, 2026-10
94/100 stars
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anti ly6g antibody  (Bio X Cell)
96
Bio X Cell anti ly6g antibody
IFNAR1 −/− Tpl2 −/− mice show the highest recruitment of neutrophils and unique overexpression of IFN-λ and CXCL1 at 7 dpi with influenza. A WT ( n = 20), Tpl2 −/− ( n = 14), Ifnar1 −/− ( n = 15), and Ifnar1 −/− Tpl2 −/− ( n = 14) mice were infected intranasally with 10 4 pfu of influenza A/x31 and euthanized at 7 dpi. The lungs were lavaged, perfused with PBS, digested with collagenase, and interstitial leukocytes were enriched by Percoll density gradient centrifugation. Neutrophils (Siglec F − CD11b high CD11c low <t>Ly6G</t> + ) in the tissue of infected mice at 7 dpi are shown. Data are pooled from 3 independent experiments. Males are represented as squares, and females are represented as circles. One-way ANOVA with Tukey’s multiple comparison test was performed with * p < 0.05, ** p < 0.01, and *** p < 0.001. WT ( n = 22), Tpl2 −/− ( n = 13), Ifnar1 −/− ( n = 19), and Ifnar1 −/− Tpl2 −/− ( n = 21) mice were infected intranasally with 10 4 pfu of influenza A/x31 and euthanized at 7 dpi. The lungs were perfused and lavaged prior to extraction and then homogenized for analysis of cytokine expression for B IFN-γ, (C) IL-6, D IL-1β, E G-CSF, F IFN-λ (IL-28), and G CXCL1. Squares represent male mice, and circles represent female mice. Data are pooled from 2 independent experiments. One-way ANOVA with Tukey’s multiple comparison test was performed with * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001. WT ( n = 17), Tpl2 −/− ( n = 16), Ifnar1 −/− ( n = 21), and Ifnar1 −/− Tpl2 −/− ( n = 13) mice were infected intranasally with 10 4 pfu of influenza A/x31 and euthanized at 7 dpi. Whole lungs (without perfusion or lavage) were homogenized for analysis of cytokine expression for H CXCL1, I IFN-γ, J IL-6, and K CCL2. Squares represent male mice, and circles represent female mice. Data are pooled from 2 independent experiments. One-way ANOVA with Tukey’s multiple comparison test was performed with * p < 0.05 and ** p < 0.01.
Anti Ly6g Antibody, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+ifn%CE%BB2+3+antibody/InVivoMAb+anti-mouse+Ly6G/pmc09558022-142-16-21
Average 96 stars, based on 1 article reviews
anti ly6g antibody - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier

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IFNAR1 −/− Tpl2 −/− mice show the highest recruitment of neutrophils and unique overexpression of IFN-λ and CXCL1 at 7 dpi with influenza. A WT ( n = 20), Tpl2 −/− ( n = 14), Ifnar1 −/− ( n = 15), and Ifnar1 −/− Tpl2 −/− ( n = 14) mice were infected intranasally with 10 4 pfu of influenza A/x31 and euthanized at 7 dpi. The lungs were lavaged, perfused with PBS, digested with collagenase, and interstitial leukocytes were enriched by Percoll density gradient centrifugation. Neutrophils (Siglec F − CD11b high CD11c low Ly6G + ) in the tissue of infected mice at 7 dpi are shown. Data are pooled from 3 independent experiments. Males are represented as squares, and females are represented as circles. One-way ANOVA with Tukey’s multiple comparison test was performed with * p < 0.05, ** p < 0.01, and *** p < 0.001. WT ( n = 22), Tpl2 −/− ( n = 13), Ifnar1 −/− ( n = 19), and Ifnar1 −/− Tpl2 −/− ( n = 21) mice were infected intranasally with 10 4 pfu of influenza A/x31 and euthanized at 7 dpi. The lungs were perfused and lavaged prior to extraction and then homogenized for analysis of cytokine expression for B IFN-γ, (C) IL-6, D IL-1β, E G-CSF, F IFN-λ (IL-28), and G CXCL1. Squares represent male mice, and circles represent female mice. Data are pooled from 2 independent experiments. One-way ANOVA with Tukey’s multiple comparison test was performed with * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001. WT ( n = 17), Tpl2 −/− ( n = 16), Ifnar1 −/− ( n = 21), and Ifnar1 −/− Tpl2 −/− ( n = 13) mice were infected intranasally with 10 4 pfu of influenza A/x31 and euthanized at 7 dpi. Whole lungs (without perfusion or lavage) were homogenized for analysis of cytokine expression for H CXCL1, I IFN-γ, J IL-6, and K CCL2. Squares represent male mice, and circles represent female mice. Data are pooled from 2 independent experiments. One-way ANOVA with Tukey’s multiple comparison test was performed with * p < 0.05 and ** p < 0.01.

Journal: Inflammation

Article Title: The Influenza-Induced Pulmonary Inflammatory Exudate in Susceptible Tpl2 -Deficient Mice Is Dictated by Type I IFN Signaling

doi: 10.1007/s10753-022-01736-8

Figure Lengend Snippet: IFNAR1 −/− Tpl2 −/− mice show the highest recruitment of neutrophils and unique overexpression of IFN-λ and CXCL1 at 7 dpi with influenza. A WT ( n = 20), Tpl2 −/− ( n = 14), Ifnar1 −/− ( n = 15), and Ifnar1 −/− Tpl2 −/− ( n = 14) mice were infected intranasally with 10 4 pfu of influenza A/x31 and euthanized at 7 dpi. The lungs were lavaged, perfused with PBS, digested with collagenase, and interstitial leukocytes were enriched by Percoll density gradient centrifugation. Neutrophils (Siglec F − CD11b high CD11c low Ly6G + ) in the tissue of infected mice at 7 dpi are shown. Data are pooled from 3 independent experiments. Males are represented as squares, and females are represented as circles. One-way ANOVA with Tukey’s multiple comparison test was performed with * p < 0.05, ** p < 0.01, and *** p < 0.001. WT ( n = 22), Tpl2 −/− ( n = 13), Ifnar1 −/− ( n = 19), and Ifnar1 −/− Tpl2 −/− ( n = 21) mice were infected intranasally with 10 4 pfu of influenza A/x31 and euthanized at 7 dpi. The lungs were perfused and lavaged prior to extraction and then homogenized for analysis of cytokine expression for B IFN-γ, (C) IL-6, D IL-1β, E G-CSF, F IFN-λ (IL-28), and G CXCL1. Squares represent male mice, and circles represent female mice. Data are pooled from 2 independent experiments. One-way ANOVA with Tukey’s multiple comparison test was performed with * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001. WT ( n = 17), Tpl2 −/− ( n = 16), Ifnar1 −/− ( n = 21), and Ifnar1 −/− Tpl2 −/− ( n = 13) mice were infected intranasally with 10 4 pfu of influenza A/x31 and euthanized at 7 dpi. Whole lungs (without perfusion or lavage) were homogenized for analysis of cytokine expression for H CXCL1, I IFN-γ, J IL-6, and K CCL2. Squares represent male mice, and circles represent female mice. Data are pooled from 2 independent experiments. One-way ANOVA with Tukey’s multiple comparison test was performed with * p < 0.05 and ** p < 0.01.

Article Snippet: They were administered either 200 μg isotype control antibody (clone 2A3#BE0089, BioXCell, red line), 200 μg anti-Ly6G antibody (clone 1A8 #BE0075-1, BioXCell, grey line), or 2.5 μg neutralizing anti-IFNλ2/3 antibody (clone 244716 #MAB17892, R&D Systems, dashed black line) intraperitoneally in 200 μl on days 5 and 7 post-infection.

Techniques: Over Expression, Infection, Gradient Centrifugation, Comparison, Extraction, Expressing

Anti-Ly6G treatment is only modestly beneficial for influenza-infected Ifnar1 −/− Tpl2 −/− mice. Mice were infected intranasally with 10 4 pfu of influenza A/x31. They were administered either 200 μg isotype control antibody (clone 2A3#BE0089, BioXCell, red line), 200 μg anti-Ly6G antibody (clone 1A8 #BE0075-1, BioXCell, grey line), or 2.5 μg neutralizing anti-IFNλ2/3 antibody (clone 244716 #MAB17892, R&D Systems, dashed black line) intraperitoneally in 200 μl on days 5 and 7 post-infection. Their weight change ( A ) and clinical scores ( B ) were recorded daily. Survival ( C ) was assessed through 12 dpi. Numbers of mice: Ifnar1 −/− Tpl2 −/− treated with isotype control ( n = 4) mice, Ifnar1 −/− Tpl2 −/− treated with anti-IFNλ2/3 ( n = 3), and Ifnar1 −/− Tpl2 −/− treated with anti-Ly6G ( n = 5). Mean and SEM are shown. Data are pooled from 2 independent experiments. Log-rank Mantel-Cox test was used for statistics.

Journal: Inflammation

Article Title: The Influenza-Induced Pulmonary Inflammatory Exudate in Susceptible Tpl2 -Deficient Mice Is Dictated by Type I IFN Signaling

doi: 10.1007/s10753-022-01736-8

Figure Lengend Snippet: Anti-Ly6G treatment is only modestly beneficial for influenza-infected Ifnar1 −/− Tpl2 −/− mice. Mice were infected intranasally with 10 4 pfu of influenza A/x31. They were administered either 200 μg isotype control antibody (clone 2A3#BE0089, BioXCell, red line), 200 μg anti-Ly6G antibody (clone 1A8 #BE0075-1, BioXCell, grey line), or 2.5 μg neutralizing anti-IFNλ2/3 antibody (clone 244716 #MAB17892, R&D Systems, dashed black line) intraperitoneally in 200 μl on days 5 and 7 post-infection. Their weight change ( A ) and clinical scores ( B ) were recorded daily. Survival ( C ) was assessed through 12 dpi. Numbers of mice: Ifnar1 −/− Tpl2 −/− treated with isotype control ( n = 4) mice, Ifnar1 −/− Tpl2 −/− treated with anti-IFNλ2/3 ( n = 3), and Ifnar1 −/− Tpl2 −/− treated with anti-Ly6G ( n = 5). Mean and SEM are shown. Data are pooled from 2 independent experiments. Log-rank Mantel-Cox test was used for statistics.

Article Snippet: They were administered either 200 μg isotype control antibody (clone 2A3#BE0089, BioXCell, red line), 200 μg anti-Ly6G antibody (clone 1A8 #BE0075-1, BioXCell, grey line), or 2.5 μg neutralizing anti-IFNλ2/3 antibody (clone 244716 #MAB17892, R&D Systems, dashed black line) intraperitoneally in 200 μl on days 5 and 7 post-infection.

Techniques: Infection, Control

The inflammatory mediator NOS2 is overexpressed in the lungs in Ifnar1 −/− Tpl2 −/− mice, with neutrophils and inflammatory monocytes redundantly contributing to its expression. WT ( n = 22), Tpl2 −/− ( n = 13), Ifnar1 −/− ( n = 19), and Ifnar1 −/− Tpl2 −/− ( n = 21) mice were infected intranasally with 10 4 pfu of influenza A/x31 and euthanized at 7 dpi. The lungs were homogenized for RNA extraction and analysis of gene expression by real-time PCR for A MPO, B NOS2, and C TNFSF10. Squares represent male mice, and circles represent female mice. Data are pooled from 2 independent experiments. One-way ANOVA with Tukey’s multiple comparison test was performed with * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001. WT ( n = 8), Tpl2 −/− ( n = 9), Ifnar1 −/− ( n = 6), and Ifnar1 −/− Tpl2 −/− ( n = 6) mice were infected intranasally with 10 4 pfu of influenza A/x31 and euthanized at 7 dpi. Their lungs were digested with collagenase, and the following leukocytes populations were sorted: D , G , J , M alveolar macrophages (AM; Siglec F high CD11b int ), E , H , K , N neutrophils (Neut; Siglec F − CD11b high CD11c low Ly6G + ), and F , I , L , O inflammatory monocytes (IM; Siglec F − CD11b high CD11c low Ly6C + ). Gene expression was analyzed by qPCR, and data were normalized for each cell type relative to the actin endogenous control and the WT strain for a given cell type as baseline, which was designated a value of 1. Data are pooled from 3 independent experiments. One-way ANOVA with Tukey’s multiple comparison test was performed. * p < 0.05 and ** p < 0.01. Female mice were used for these experiments.

Journal: Inflammation

Article Title: The Influenza-Induced Pulmonary Inflammatory Exudate in Susceptible Tpl2 -Deficient Mice Is Dictated by Type I IFN Signaling

doi: 10.1007/s10753-022-01736-8

Figure Lengend Snippet: The inflammatory mediator NOS2 is overexpressed in the lungs in Ifnar1 −/− Tpl2 −/− mice, with neutrophils and inflammatory monocytes redundantly contributing to its expression. WT ( n = 22), Tpl2 −/− ( n = 13), Ifnar1 −/− ( n = 19), and Ifnar1 −/− Tpl2 −/− ( n = 21) mice were infected intranasally with 10 4 pfu of influenza A/x31 and euthanized at 7 dpi. The lungs were homogenized for RNA extraction and analysis of gene expression by real-time PCR for A MPO, B NOS2, and C TNFSF10. Squares represent male mice, and circles represent female mice. Data are pooled from 2 independent experiments. One-way ANOVA with Tukey’s multiple comparison test was performed with * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001. WT ( n = 8), Tpl2 −/− ( n = 9), Ifnar1 −/− ( n = 6), and Ifnar1 −/− Tpl2 −/− ( n = 6) mice were infected intranasally with 10 4 pfu of influenza A/x31 and euthanized at 7 dpi. Their lungs were digested with collagenase, and the following leukocytes populations were sorted: D , G , J , M alveolar macrophages (AM; Siglec F high CD11b int ), E , H , K , N neutrophils (Neut; Siglec F − CD11b high CD11c low Ly6G + ), and F , I , L , O inflammatory monocytes (IM; Siglec F − CD11b high CD11c low Ly6C + ). Gene expression was analyzed by qPCR, and data were normalized for each cell type relative to the actin endogenous control and the WT strain for a given cell type as baseline, which was designated a value of 1. Data are pooled from 3 independent experiments. One-way ANOVA with Tukey’s multiple comparison test was performed. * p < 0.05 and ** p < 0.01. Female mice were used for these experiments.

Article Snippet: They were administered either 200 μg isotype control antibody (clone 2A3#BE0089, BioXCell, red line), 200 μg anti-Ly6G antibody (clone 1A8 #BE0075-1, BioXCell, grey line), or 2.5 μg neutralizing anti-IFNλ2/3 antibody (clone 244716 #MAB17892, R&D Systems, dashed black line) intraperitoneally in 200 μl on days 5 and 7 post-infection.

Techniques: Expressing, Infection, RNA Extraction, Gene Expression, Real-time Polymerase Chain Reaction, Comparison, Control